车叶草苷酸

中文名称:

车叶草苷酸

中文同义词:

车叶草甙酸;车叶草苷酸;车叶草苷酸标准品;车叶草苷酸对照品

英文名称:

asperulosidic acid

英文同义词:

asperulosidic acid;(1S,4aS,5S,7aS)-7-[(Acetyloxy)methyl]-1-(beta-D-glucopyranosyloxy)-1,4a,5,7a-tetrahydro-5-hydroxycyclopenta[c]pyran-4-carboxylic acid;Cyclopenta[c]pyran-4-carboxylic acid, 7-[(acetyloxy)methyl]-1-(β-D-glucopyranosyloxy)-1,4a,5,7a-tetrahydro-5-hydroxy-, (1S,4aS,5S,7aS)-;Asperulosidic acid >=90% (LC/MS-ELSD)

CAS号:

25368-11-0

分子式:

C18H24O12

分子量:

432.38

EINECS号:

相关类别:

标准品;对照品;中药对照品;植物提取物;标准品-对照品;分析试剂标准品;标准品 -中药标准品;标准品，对照品

Mol文件:

25368-11-0.mol

沸点 

715.6±60.0 °C(Predicted)

密度 

1?+-.0.1 g/cm3(Predicted)

储存条件 

?20°C

酸度系数(pKa)

4.32±0.70(Predicted)

作用

车叶草苷酸是环烯醚萜类物质，是诺尼果汁中重要的活性成分，具有抗肿瘤、抗病毒、抗氧化、抗畸变等活性，与人体健康密切相关。

制备

取10kg白花蛇舌草加入10倍量体积浓度为75％乙醇回流提取2次，每次1h，60℃减压浓缩至相对密度为1.10-1.20；往浓缩液中加入重量百分比为0.5％的活性炭脱色，高速离心，取上清，真空干燥，得白花蛇舌草粗提物；将粗提物采用30％甲醇溶解，以ODS开口柱为固定相，30％甲醇水溶液洗脱，回收甲醇后，真空干燥。干燥后样品采用5％甲醇溶解，采用高速逆流色谱法分离获得车叶草苷酸。制备条件：YMC-PackODS-A色谱柱(20×250mm，5μm)为色谱柱，以体积浓度为5％的甲醇为流动相，等梯度洗脱60min；检测波长235nm；进样体积100μL。

生物活性

Asperulosidic Acid (ASPA) 车叶草苷酸是一种具有生物活性的环烯醚萜苷，从白花蛇舌草 (Hedyotis diffusa Willd) 的草药中提取的。 Asperulosidic Acid (ASPA) 具有抗肿瘤，抗氧化和抗炎作用。 Asperulosidic Acid (ASPA) 通过抑制 NF-κB 和丝裂原活化蛋白激酶 (MAPK) 信号通路抑制炎性细胞因子(TNF-α，IL-6) 释放发挥抗炎作用。

体外研究

Asperulosidic Acid (ASPA) (40-160 μg/mL; pre- 1 hour) significantly down-regulates the mRNA levels of TNF-α and IL-6 in LPS-induced RAW 264.7 cells compared with the group treated with LPS alone . Asperulosidic Acid (ASPA) (40-160 μg/mL; pre- 1 hour) decreases IκB-α phosphorylation in a concentration-dependent manner, decreases Erk1/2 phosphorylation at all concentration levels, but there was no effect on p-p38 . RT-PCR Cell Line: RAW 264.7 cells Concentration: 40 μg/mL, 80 μg/mL, and 160 μg/mL Incubation Time: Pre-treatment 1 hour Result: Decreased TNF-α and IL-6 mRNA expression. Western Blot Analysis Cell Line: RAW 264.7 cells Concentration: 40 μg/mL, 80 μg/mL, and 160 μg/mL Incubation Time: Pre-treatment 1 hour Result: Decreased IκB-α phosphorylation and Erk1/2 phosphorylation.

Cell Line:

RAW 264.7 cells

Concentration:

40 μg/mL, 80 μg/mL, and 160 μg/mL

Incubation Time:

Pre-treatment 1 hour

Result:

Decreased TNF-α and IL-6 mRNA expression.

Cell Line:

RAW 264.7 cells

Concentration:

40 μg/mL, 80 μg/mL, and 160 μg/mL

Incubation Time:

Pre-treatment 1 hour

Result:

Decreased IκB-α phosphorylation and Erk1/2 phosphorylation.

WGK Germany 

3