中文名称:

银杏素

中文同义词:

银杏双黄酮/白果双黄酮;银杏双黄酮, 来源于银杏叶;GINKGETIN 银杏双黄酮;白果双黄酮(银杏双黄酮,银杏黄素,银杏素);白果双黄酮,银杏双黄酮,银杏黄素;银杏黄酮甙;银杏双黄酮,银杏黄素,银杏素;白果双黄酮(银杏双黄酮

英文名称:

GINKGETIN

英文同义词:

GINKGETIN;4,5,5,7-Tetrahydroxy-4,7-dimethoxy-8,3-biflavone;5,7-Dihydroxy-8-[2-methoxy-5-(5-hydroxy-7-methoxy-4-oxo-4H-1-benzopyran-2-yl)phenyl]-2-(4-hydroxyphenyl)-4H-1-benzopyran-4-one;Ginkgetin, >98%;5,7-Dihydroxy-8-[5-(5-hydroxy-7-methoxy-4-oxo-4H-1-benzopyran-2-yl)-2-methoxyphenyl]-2-(4-hydroxyphenyl)-4H-1-benzopyran-4-one;GINKGETIN(RG)(PLEASE CALL);4H-1-Benzopyran-4-one,5,7-dihydroxy-8-[5-(5-hydroxy-7-methoxy-4-oxo-4H-1-benzopyran-2-yl)-2-methoxyphenyl]-2-(4-hydroxyphenyl)-;Ginkgetin, 98%, from Ginkgo biloba L.

CAS号:

481-46-9

分子式:

C32H22O10

分子量:

566.51

EINECS号:

相关类别:

中草药成分;中药对照品;标准品;对照品;植物提取物;分析试剂标准品;对照品-中药对照品;黄酮;标准品 -中药标准品;标准品，对照品

Mol文件:

481-46-9.mol

熔点 

297 °C(Solv: acetone (67-64-1))

沸点 

863.7±65.0 °C(Predicted)

密度 

1.506±0.06 g/cm3(Predicted)

酸度系数(pKa)

6.18±0.40(Predicted)

生物活性

Ginkgetin 是一种从银杏叶中分离得到的双黄酮。Ginkgetin 具有抗肿瘤，抗炎，神经保护，抗真菌的作用。Ginkgetin 也是一种有效的 Wnt 信号抑制剂，IC50 值为 5.92 μM。

靶点

Wnt 5.92 μM (IC 50 )

Wnt 5.92 μM (IC 50 )

体外研究

Ginkgetin (2.5-20 μM; 48 h) inhibits the growth of Daoy and D283 cell lines, and induces G 2 /M cell cycle arrest in Daoy cells. Ginkgetin (20-40 μM; 24 h) significantly activates the apoptosis of osteosarcoma cells in a concentration-dependent manner. Ginkgetin (10-20 μM; 3-24 h) down-regulated the expression of Wnt target genes without affecting the expression of β-catenin in medulloblastoma cells. Ginkgetin (1-10 μM; 24 or 48 h) significantly inhibits the VEGF-induced endothelial cell proliferation, migration, and wound recovery in a concentration-dependent manner. Ginkgetin (5-10 μM; 48 h) induces autophagy responsible for cell death in A549. Cell Viability Assay Cell Line: Daoy and D283 cell lines Concentration: 2.5, 5, 10, 20 μM Incubation Time: 48 hours Result: Inhibited the cell growth, with IC 50 s of 14.65 and 15.81 μM for Daoy and D283 cells, respectively. Apoptosis Analysis Cell Line: Osteosarcoma cells Concentration: 20, 30, 40 μM Incubation Time: 24 hours Result: Markedly induced the apoptosis of osteosarcoma cells in a concentration-dependent manner. Cell Cycle Analysis Cell Line: Daoy cells Concentration: 2.5, 5, 10, 20 μM Incubation Time: 24 hours Result: Increased G 2 /M phase, compared with that of control, indicating a G 2 /M cell phase arrest. Cell Cycle Analysis Cell Line: Daoy and D283 cell lines Concentration: 10, 20 μM Incubation Time: 3, 6, 12, 24 hours Result: Attenuated the expression of Wnt target genes, Axin2, cyclin D1 and survivin at 20 μM for 24 h in Daoy cells. Unaffected the level of total β-catenin and diminished the level of β-catenin phosphorylation in a time- and concentration-dependent manner.

Cell Line:

Daoy and D283 cell lines

Concentration:

2.5, 5, 10, 20 μM

Incubation Time:

48 hours

Result:

Inhibited the cell growth, with IC 50 s of 14.65 and 15.81 μM for Daoy and D283 cells, respectively.

Cell Line:

Osteosarcoma cells

Concentration:

20, 30, 40 μM

Incubation Time:

24 hours

Result:

Markedly induced the apoptosis of osteosarcoma cells in a concentration-dependent manner.

Cell Line:

Daoy cells

Concentration:

2.5, 5, 10, 20 μM

Incubation Time:

24 hours

Result:

Increased G 2 /M phase, compared with that of control, indicating a G 2 /M cell phase arrest.

Cell Line:

Daoy and D283 cell lines

Concentration:

10, 20 μM

Incubation Time:

3, 6, 12, 24 hours

Result:

Attenuated the expression of Wnt target genes, Axin2, cyclin D1 and survivin at 20 μM for 24 h in Daoy cells. Unaffected the level of total β-catenin and diminished the level of β-catenin phosphorylation in a time- and concentration-dependent manner.

体内研究

Ginkgetin (25-100 mg/kg; i.p. 2 hours after the onset of ischemia) exerts anti-inflammatory effects on cerebral ischemia/reperfusion-induced injury in a rat model via the TLR4/NF-κB signaling pathway. Ginkgetin (30 mg/kg; intragastrically once per day for 42 d) suppresses tumor growth in A549 cells bearing nude mice. Animal Model: Male Sprague-Dawley rats (200-220 g) Dosage: 25, 50, 100 mg/kg Administration: I.p. 2 hours after the onset of ischemia Result: Reduced the neurological deficit score. Suppressed the expression of NF-κB, TLR4 and IκBαin ischemic penumbra cortex, and inhibited the degradation of IκBα. Decreased the expressions of ICAM-1, COX-2, and iNOS. Downregulated downstream inflammatory factor PGE2 and TNF-α expression. Decreased IL-1β, IL-6, IL-8, and IL-10 protein expression.

Animal Model:

Male Sprague-Dawley rats (200-220 g)

Dosage:

25, 50, 100 mg/kg

Administration:

I.p. 2 hours after the onset of ischemia

Result:

Reduced the neurological deficit score. Suppressed the expression of NF-κB, TLR4 and IκBαin ischemic penumbra cortex, and inhibited the degradation of IκBα. Decreased the expressions of ICAM-1, COX-2, and iNOS. Downregulated downstream inflammatory factor PGE2 and TNF-α expression. Decreased IL-1β, IL-6, IL-8, and IL-10 protein expression.

化学性质 

可溶于甲醇、乙醇、DMSO等有机溶剂，来源于银杏。

用途 

银杏双黄酮具有调血脂，降胆固醇，治疗心绞痛的作用。

用途 

用于含量测定/鉴定/药理实验等