尼罗红

中文名称:

尼罗红

中文同义词:

尼罗红, 用于荧光分析;尼罗红5H-BENZO[A]PHENOXAZIN-5-ONE,9-(DIETHYLAMINO)-;Nile Red, 用于荧光分析,≥98.0% (HPLC);尼罗红, 用于荧光分析,≥98.0% (HPLC);Nile Red, 用于荧光分析,≥95.0% (HPLC);尼罗红, 用于荧光分析,≥95.0% (HPLC);尼罗红/尼罗丝/NILE RED;尼罗红 500MG

英文名称:

NILE RED

英文同义词:

NILE BLUE A OXAZONE;NILE RED;9-(DIETHYLAMINO)-5H-BENZO[A]PHENOXAZIN-5-ONE;9-(diethylamino)-5h-benzo[a]phenoxazin-5-on;nile;9-(diethylamino)benzo[a]phenoxazin-5(5H)-one;FLUORESCENCE QY-STANDARD E CERTIFIED;Fluorescent Spectral Standard E, certified

CAS号:

7385-67-3

分子式:

C20H18N2O2

分子量:

318.37

EINECS号:

230-966-0

相关类别:

化工原料;生化试剂-色素类;其他生化试剂;色素类;生化试剂;荧光探针，标记，颗粒和染色;marker;Electroluminescence;Functional Materials

Mol文件:

7385-67-3.mol

熔点 

203-205 °C(lit.)

沸点 

457.53°C (rough estimate)

密度 

1.1871 (rough estimate)

折射率 

1.5700 (estimate)

储存条件 

2-8°C

储存条件 

room temp

溶解度 

methanol: soluble1mg/mL

酸度系数(pKa)

4.08±0.20(Predicted)

形态

Powder

颜色

Dark red to maroon

BRN 

279110

稳定性

Stable. Incompatible with strong oxidizing agents.

InChIKey

VOFUROIFQGPCGE-UHFFFAOYSA-N

CAS 数据库

7385-67-3(CAS DataBase Reference)

EPA化学物质信息

5H-Benzo[a]phenoxazin-5-one, 9-(diethylamino)- (7385-67-3)

生物活性

Nile Red (Nile Blue A oxazone, Phenoxazone 9)是一种十分有效的荧光染料，可在疏水环境下检测细胞内脂质滴 intracellular lipid droplets。Nile Red 可用于细胞内脂质，蛋白质的疏水域和溶酶体磷脂内含体的染色。

靶点

Target Value lipid droplet ()

Target

Value

lipid droplet ()

体外研究

Nile red-stained, lipid droplet-filled macrophages exhibit greater fluorescence intensity than does Nile red-stained control macrophages, and the two cell populations could be differentiated and analyzed by flow cytofluorometry. Better selectivity for cytoplasmic lipid droplets is obtained when the cells are viewed for yellow-gold fluorescence (excitation, 450-500 nm; emission, greater than 528 nm) rather than red fluorescence (excitation, 515-560 nm; emission, greater than 590 nm). Nile red is strongly fluorescent, but only in the presence of a hydrophobic environment. Nile red is very soluble in the lipids it is intended to show, and it does not interact with any tissue constituent except by solution. Spectral and physicochemical properties of the lipophilic dye Nile red induce a yellow-gold-spectral shift in its excitation-emission peak, allowing it to fluoresce in the green emission spectrum only when in a lipid-rich environment, but not in more polar environments.

体内研究

When Nile red-stained Caenorhabditis elegans is viewed for green fluorescence, discrete lipid bodies can be observed throughout the intestine and other tissues either in clusters or evenly dispersed, depending on the animals genotype or experimental treatment.

用途 

生化研究

安全说明 

24/25

WGK Germany 

3

F 

8-10

海关编码 

29349990